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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has become observed in many tumors, which leads to chromosome instability, and therefore could contribute to tumorigenesis. This protein was uncovered to affiliate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in cell-cycle regulated histone gene expression and performs a important purpose in advertising and marketing cell-cycle development inside the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, are already described. Two more splice variants had been reported but specific nucleotide sequence details is not still accessible.
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Blocking peptides are peptides that bind especially to your target antibody and block antibody binding. These peptide normally has the epitope regarded by the antibody.
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This manual outlines further probable optimization of mobile and immunoassay parameters to make certain the absolute best results are...
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This manual outlines more possible optimization of mobile and immunoassay parameters to ensure the best possible final results are...
Antibodies bound to the blocking peptide not bind for the epitope about the target protein. By evaluating the staining within the blocked antibody vs . the antibody by yourself, one can see which staining is particular.
Target Information Cyclin E belongs to your remarkably conserved cyclin relatives, whose customers are characterized by a extraordinary periodicity in protein abundance throughout the cell cycle. Cyclins purpose as regulators of CDK kinases. Diverse cyclins exhibit unique expression and degradation patterns which lead towards the temporal coordination of each and every mitotic event. Cyclin E sorts a complex with and capabilities as being a regulatory subunit of CDK2, whose exercise is required for cell cycle G1/S transition. This protein accumulates within the G1-S stage boundary which is degraded as cells development by S phase.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both equally antibodies...
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